MicroRNA-29a in Osteoblasts Represses High-Fat Diet-Mediated Osteoporosis and Body Adiposis through Targeting Leptin
نویسندگان
چکیده
Skeletal tissue involves systemic adipose metabolism and energy expenditure. MicroRNA signaling controls high-fat diet (HFD)-induced bone fat homeostasis dysregulation remains uncertain. This study revealed that transgenic overexpression of miR-29a under control osteocalcin promoter in osteoblasts (miR-29aTg) attenuated HFD-mediated body overweight, hyperglycemia, hypercholesterolemia. HFD-fed miR-29aTg mice showed less mass loss, fatty marrow, visceral together with increased subscapular brown than wild-type mice. HFD-induced O2 underconsumption, respiratory quotient repression, heat underproduction were In vitro, repressed transcriptomic landscapes the adipocytokine pathway, acid metabolism, lipid transport, etc., marrow mesenchymal progenitor cells. Forced expression promoted osteogenic differentiation but inhibited adipocyte formation. brown/beige markers Ucp-1, Pgc-1α, P2rx5, Pat2 white Tcf21 Hoxc9 expression. The microRNA also reduced peroxisome formation leptin during downregulated tissue. Taken together, controlled cells to preserve anabolism, which reversed underutilization overproduction. sheds light on a new molecular mechanism by integrity counteracts whole-body
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ژورنال
عنوان ژورنال: International Journal of Molecular Sciences
سال: 2021
ISSN: ['1661-6596', '1422-0067']
DOI: https://doi.org/10.3390/ijms22179135